Internal standard for Western Blotting

Melissa Greeve gliftor at hotmail.com
Thu Sep 6 21:53:27 EST 2001


Hi Silas

Occasionally when I was looking at proteins about the same size as
B-actin (or two proteins of similar size), I would save the blot after
probing for the protein of interest and reincubate it with
anti-B-actin. Sometimes it worked fine, but you have to consider that
there may be some loss of protein during the washes, and hence
sometimes the blots are blotchy.

-M




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