Troubleshooting with the second dimension of 2D gel

Thomas, Vincent vincent.thomas at ic.ac.uk
Tue Sep 18 04:09:06 EST 2001


Dear all, 

We are currently attempting to set up a proteomics project in my lab.... and
are experiencing trouble in trying to separate the focused proteins. The
problem is as follow: The separation of the protein in the 15% bis acryl gel
is fine until the 3 to 4 last centimeters of the gel where we totaly lose
resolution, i.e., no more dots but only a massive smir/vertical
streaking..... any idea of what is going on?????

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