Troubleshooting with the second dimension of 2D gel

Elias Klein e0klei01 at
Wed Sep 19 13:25:13 EST 2001

Are you silver staining or using Sypro?

"Thomas, Vincent" wrote:

> Dear all,
> We are currently attempting to set up a proteomics project in my lab.... and
> are experiencing trouble in trying to separate the focused proteins. The
> problem is as follow: The separation of the protein in the 15% bis acryl gel
> is fine until the 3 to 4 last centimeters of the gel where we totaly lose
> resolution, i.e., no more dots but only a massive smir/vertical
> streaking..... any idea of what is going on?????
> ---

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