Troubleshooting with the second dimension of 2D gel

[Elias Klein]

Are you silver staining or using Sypro?

"Thomas, Vincent" wrote:

> Dear all,
>
> We are currently attempting to set up a proteomics project in my lab.... and
> are experiencing trouble in trying to separate the focused proteins. The
> problem is as follow: The separation of the protein in the 15% bis acryl gel
> is fine until the 3 to 4 last centimeters of the gel where we totaly lose
> resolution, i.e., no more dots but only a massive smir/vertical
> streaking..... any idea of what is going on?????
>
> ---