2D protein standards

Shinichiro Wachi swachi at mailbox.ucdavis.edu
Fri Sep 21 11:24:29 EST 2001


Which molecular weight standards do you recommend for 2DGE?  Currently I
am using silver staining, with 10% or 9% SDS PAGE.

Thanks,
Shin

On Thu, 6 Sep 2001, Mitchell Isaacs wrote:

> "Dawn McLellan" <Dawn.McLellan at TCH.Harvard.edu> wrote in message
> news:3B97EC5A.3A0CAA35 at tch.harvard.edu...
> > Has any one been successful in running molecular weight standards for 2D
> > gels?
> Yep - you load them onto your gel in a separate lane when you're doing your
> second dimension. Many precast 2D gels have a marker lane.
>
> Cheers,
> Mitchell
>
>
>




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