protein self association - buffer effect

Francoise Martz francoise.martz at metla.fi
Thu Sep 27 03:11:00 EST 2001


Hi there,

A plant protein was expressed in E.coli with a His tag and purified it 
using Ni-NTA spin column. On native PAGE, the protein appeared as oligomers 
in presence of tris or phosphate buffer, but appeared as monomer in 
presence of hepes or mops buffer (all at same pH).
A similar (but weaker) self association was detected using plant crude 
extract (no His tag).

Not a biochemist, I would be pleased to have any comment about the meaning 
of these differences in oligomerization in different buffer conditions ?
Thanks for your help!

francoise.martz at metla.fi



___________________________________
Françoise Martz
The Finnish Forest Research Institute
Rovaniemi Research Station
P.O. Box 16
96301 Rovaniemi, Finland
Tel: +358 16 336 4325 - Fax: +358 16 336 4640

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