HELP: strange c-terminal deletion casue protein run high in SDS-PAGE

andor maildie at yahoo.com
Sat Sep 29 23:43:37 EST 2001


My protein(CMV-Myc Vector) is around 15KD in a standard 15% SDS-PAGE.
To argue a question with my classmates:
In a 15% SDS-PAGE,could protein with one amino acid difference be
detected?
I deleted some amino acids at C-terminal in my protein on purpose.

When I deleted the last amino acid in C-terminal, the protein "Del-1"
run different and to our surprise HIGHER than the full-length protein,
deleted the last 2 and three amino acid get the same band and both run
HIGHER than the Del-1.

I have read some posts on this newsgroups ,learned that protein may
sometimes show a un normal result in SD-PAGE, and to my knowledge one
amino acid difference can not be detected in a 15% SDS-PAGE,however I
just can not come up with even a reasonable hypothesis for my strange
result above?

Are there someone meet the same question with me? I need help!




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