Ni-NTA biosensor chip?

John s330477 at student.uq.edu.au
Sun Apr 7 19:49:54 EST 2002


Hi,

I am interested if anyone has used a Ni-NTA biosensor chip to immobilise a
His-tagged protein then analysed further protein:protein interactions with
the His-tagged protein?

We have an IAsys instrument, but I don't think they have a Ni-NTA chip.  I
could probably get access to a BIACORE if necessary.

Any opinions/experiences/recommendations would be useful.

Also source and approximate cost of the Ni-NTA chips used would be also much
appreciated.

Thanks,
John.





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