IPA precipitation of virus

Morten Kring mortenkring at hotmail.com
Tue Apr 16 05:41:45 EST 2002


I'm looking for help and hints on how to remove virus from a
ionexchanger eluate containing blood plasma proteins. In particular i'm
looking for methods
to remove MVM (minute virus og mice) which is a part of the Parviviridiae
familiy and is especially
difficult to handle.
I was thinking of doing a fractionated precipitation with IPA
(isopropyl-alcohol or isopropanol) at around 25 % vol./vol. IPA. Does anyone
know if this could remove or inactivate the virus at this relatively low
concentration of organic solvent?

Looking forward to any response.
Morten





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