IPA precipitation of virus

D.K. dk at no.email.thankstospam.net
Tue Apr 16 07:14:52 EST 2002


"Morten Kring" <mortenkring at hotmail.com> wrote:
>I'm looking for help and hints on how to remove virus from a
>ionexchanger eluate containing blood plasma proteins. In particular i'm
>looking for methods
>to remove MVM (minute virus og mice) which is a part of the Parviviridiae
>familiy and is especially
>difficult to handle.
>I was thinking of doing a fractionated precipitation with IPA
>(isopropyl-alcohol or isopropanol) at around 25 % vol./vol. IPA. Does anyone
>know if this could remove or inactivate the virus at this relatively low
>concentration of organic solvent?

What about low % of ammonium sulphate? Viruses should salt out
readily. 

DK




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