IPA precipitation of virus

Morten Kring mortenkring at hotmail.com
Tue Apr 16 08:07:06 EST 2002


That is of course an alternative. But what about pH of the solution ? and
roughly how low is low % ammonium sulphate?

"D.K." <dk at no.email.thankstospam.net> wrote in message
news:a9h4k0$qb7$1 at news.doit.wisc.edu...
> "Morten Kring" <mortenkring at hotmail.com> wrote:
> >I'm looking for help and hints on how to remove virus from a
> >ionexchanger eluate containing blood plasma proteins. In particular i'm
> >looking for methods
> >to remove MVM (minute virus og mice) which is a part of the Parviviridiae
> >familiy and is especially
> >difficult to handle.
> >I was thinking of doing a fractionated precipitation with IPA
> >(isopropyl-alcohol or isopropanol) at around 25 % vol./vol. IPA. Does
anyone
> >know if this could remove or inactivate the virus at this relatively low
> >concentration of organic solvent?
>
> What about low % of ammonium sulphate? Viruses should salt out
> readily.
>
> DK





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