IPA precipitation of virus

D.K. dk at no.email.thankstospam.net
Tue Apr 16 19:10:10 EST 2002


"Morten Kring" <mortenkring at hotmail.com> wrote:
>That is of course an alternative. But what about pH of the solution ? and
>roughly how low is low % ammonium sulphate?

pH is neutral (and preferably well-buffered as AS acidifies a bit)
and I would start try 5, 10, 15, 20% saturation as proteins do 
not precipitate significantly at this range. 


>"D.K." <dk at no.email.thankstospam.net> wrote in message
>news:a9h4k0$qb7$1 at news.doit.wisc.edu...
>> "Morten Kring" <mortenkring at hotmail.com> wrote:
>> >I'm looking for help and hints on how to remove virus from a
>> >ionexchanger eluate containing blood plasma proteins. In particular i'm
>> >looking for methods
>> >to remove MVM (minute virus og mice) which is a part of the Parviviridiae
>> >familiy and is especially
>> >difficult to handle.
>> >I was thinking of doing a fractionated precipitation with IPA
>> >(isopropyl-alcohol or isopropanol) at around 25 % vol./vol. IPA. Does
>anyone
>> >know if this could remove or inactivate the virus at this relatively low
>> >concentration of organic solvent?
>>
>> What about low % of ammonium sulphate? Viruses should salt out
>> readily.
>>
>> DK
>
>




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