Purification of GST-fusion protein

Chan Shzu Wei mdccsw at nus.edu.sg
Tue Apr 30 02:27:18 EST 2002


Dear colleagues,

I have problem in purifying my protein of interest  from the GST from
using GST column. I have cut my protein of interest (~25.7kDa) from the
GST fusion protein (26 kDa) with thrombin protease, and as in theory, I
should get my protein of interest in flow through, but it didn't. I
found that it came out together with the GST when I eluted with the GST
elution buffer. Please give some suggestion to purify my protein of
interest from the GST protein. Thanks.

Joyce




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