Solubility Study of Truncated Proteins

[Elsy Benjamin]

Dear List members,
It would be a great help if any one can give me insight on solubilty study
of expresssed protein.

What techniques can be used for such studies: expression cloning like
GateWay,  Invitro trancription translation?

Are there any published information about generating truncations in a
complete ORF followed by cloning and study the expression to see which
truncated protein get expressed to highest level?
 
Elsy..


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