Solubility Study of Truncated Proteins

Elsy Benjamin benjaminelsy at hotmail.com
Tue Apr 30 03:50:49 EST 2002


Dear List members,
It would be a great help if any one can give me insight on solubilty study
of expresssed protein.

What techniques can be used for such studies: expression cloning like
GateWay,  Invitro trancription translation?

Are there any published information about generating truncations in a
complete ORF followed by cloning and study the expression to see which
truncated protein get expressed to highest level?
 
Elsy..


<http://www.biowww.net/forum/read.php?f=1&i=5400&t=5400>




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