2D: disappearance

Philipp Wechner philipp.wechner at uibk.ac.at
Tue Aug 6 05:32:46 EST 2002


why do you post this agein?

Anne-Françoise Marchand schrieb:

> Hi,
> Two years ago, I have done about 50 2D gels (silver staining +
> glutaraldehyde). After analyse, we have fond one very interesting
> spot. Thus we decided to do the same maps (same protocol) with the same
> sample frozen at -80°C. The spot had disappeard!
>
> How can you explain that? Do you have any solution to find again the
> spot? Do you have a protocol to extract a spot from a silver staining
> gel with glut. (I shoud analysed the spot with a Q-TOF)? How can I know
> the name of this spot?
>
> Thanks for your ideas
> Anne-Françoise
> anne-francoise at umh.ac.be




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