SDS-PAGE for purity

steven.leonard1 Steven.leonard1 at
Tue Aug 6 11:20:32 EST 2002

You can not use any single test to claim purity. SDS-PAGE is particularly
vulnerable. I would advise using several separation methods to establish
purity e.g. chromatography, capillary electrophoresis, Acid-PAGE/SDS-PAGE
<drabena at> wrote in message
news:20020716143214.1577.qmail at
> I am currently running SDS-PAGE's to calculate the "percent area" of a
single protein.  Can anyone tell me how to run this assay to determine the
percent purity?

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