SDS PAGE optimization - defomed lanes

steven.leonard1 Steven.leonard1 at ntlworld.com
Tue Aug 6 11:23:38 EST 2002


Which plant sample are you running and which part of the plant is it from?
<monsa at ufl.edu> wrote in message
news:20020722181708.22695.qmail at ww02.hostica.com...
> Hi,
> when I run some plant protein samples (especially different cell fractions
extracts) on 10% SDS-PAGE some lanes appear wider than others. The bands
become deformed. Does anybody know how I can fix this problem? I assume
there must be a problem related to the density, or may be to the different
protein content of the diferent extracts. Glycerol may also be contributing
to this deformation, since I also have this problem when I load samples
stored in 30% glycerol.
>  I would also appreciate is you can advice me as which the best loading
buffer to use for plant protein samples is.
> Regards,
> Gonzalo
>
>
>
>
http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0


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