western blotting and white bands

Philipp Pagel p.pagel at gsf.de
Wed Aug 7 09:20:56 EST 2002


Emir Khatipov <khatipovNO at nouchicago.edu> wrote:
> Too much protein in the band is the reason. 

Yes.

> I believe it happens because too
> much luminescence bleaches the X-ray film. 

That wouldn't work - the band would still be black. The reason is the
rapid local depletion of the chemoluminescence substrate caused by huge
amounts of the HRP (or whatever enzyme you use) resulting in a negative
band which is sometimes surounded by a dark rim where the protein
concentration drops.

cu
	Philipp


-- 
Dr. Philipp Pagel                                    Tel.  +49-89-3187-3675
Institute for Bioinformatics / MIPS                  Fax.  +49-89-3187-3585
GSF - National Research Center for Environment and Health
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85764 Neuherberg
Germany



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