2D: disappearance

Jean-Etienne Poirrier jepoirrier at nospam.student.ulg.ac.be
Thu Aug 8 08:16:54 EST 2002


Hello,

Perhaps you could ask this same question in the Amersham 2D forum. Reiner 
Westermeier and Sjouke Hoving often answer quickly.

Jean-Etienne


anne-francoise.marchand at umh.ac.be (Anne-Françoise Marchand) wrote in 
news:3D4A3EB6.A224B2C6 at umh.ac.be:

> Hi,
> Two years ago, I have done about 50 2D gels (silver staining +
> glutaraldehyde). After analyse, we have fond one very interesting
> spot. Thus we decided to do the same maps (same protocol) with the same
> sample frozen at -80°C. The spot had disappeard!
> 
> How can you explain that? Do you have any solution to find again the
> spot? Do you have a protocol to extract a spot from a silver staining
> gel with glut. (I shoud analysed the spot with a Q-TOF)? How can I know
> the name of this spot?
> 
> Thanks for your ideas
> Anne-Françoise
> anne-francoise at umh.ac.be
> 



More information about the Proteins mailing list