2D gel: spot disappearance

[Jean-Etienne Poirrier]

Hello,

Perhaps you could ask this same question in the Amersham 2D forum. Reiner 
Westermeier and Sjouke Hoving often answer quickly.

Jean-Etienne

anne-francoise.marchand at umh.ac.be (Anne-Françoise Marchand) wrote in 
news:3D40FE45.94064027 at umh.ac.be:

> Hi,
> Two years ago, I have done about 50 2D gels (silver staining  +
> glutaraldehyde).  After analyse, we have fond one very interesting spot.
> Thus we decided to do the same maps (same protocol) with the same sample
> frozen at -80°C. The spot had disappeard!
> 
> How can you explain that?  Do you have any solution to find again the
> spot? Do you have a protocol to extract a spot from a silver staining
> gel with glut. (I shoud analysed the spot with a Q-TOF!)?
> How can I know the name of this spot?
> 
> Thanks for your ideas.
> Anne-Françoise.
> anne-francoise.marchand at umh.ac.be