2D gel: spot disappearance
jepoirrier at nospam.student.ulg.ac.be
Thu Aug 8 08:15:34 EST 2002
Perhaps you could ask this same question in the Amersham 2D forum. Reiner
Westermeier and Sjouke Hoving often answer quickly.
anne-francoise.marchand at umh.ac.be (Anne-Françoise Marchand) wrote in
news:3D40FE45.94064027 at umh.ac.be:
> Two years ago, I have done about 50 2D gels (silver staining +
> glutaraldehyde). After analyse, we have fond one very interesting spot.
> Thus we decided to do the same maps (same protocol) with the same sample
> frozen at -80°C. The spot had disappeard!
> How can you explain that? Do you have any solution to find again the
> spot? Do you have a protocol to extract a spot from a silver staining
> gel with glut. (I shoud analysed the spot with a Q-TOF!)?
> How can I know the name of this spot?
> Thanks for your ideas.
> anne-francoise.marchand at umh.ac.be
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