sticky protein

D.K. dk at
Fri Aug 9 15:52:26 EST 2002

Lisa Gabrielle Defazio <ldefazio at> wrote:
>Does anyone have suggestions on how to prevent my protein from sticking to
>the membrane when I dialyze it?  I'm already adding loads of BSA, and my
>buffer contains 10% glycerol.

Add low CMC detergent like Triton X-100, Tween 80, etc. 
Assuming it does not stick specifically, "preblock" membrane 
at room temp with BSA or milk proteins. 
Assuming it does, try changing membrane type. 


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