sticky protein

D.K. dk at no.email.thankstospam.net
Fri Aug 9 15:52:26 EST 2002


Lisa Gabrielle Defazio <ldefazio at stanford.edu> wrote:
>
>Does anyone have suggestions on how to prevent my protein from sticking to
>the membrane when I dialyze it?  I'm already adding loads of BSA, and my
>buffer contains 10% glycerol.

Add low CMC detergent like Triton X-100, Tween 80, etc. 
Assuming it does not stick specifically, "preblock" membrane 
at room temp with BSA or milk proteins. 
Assuming it does, try changing membrane type. 

DK



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