sticky protein

Artem Evdokimov AEVDOKIMOZ at cinci.rr.com
Fri Aug 9 21:27:10 EST 2002


Detergents, nonionic for preference. BOG sometimes works miracles, but it is
just one out of thousands of other possibilities. Watch out for micelle
overconcentration.

A.G.E.
"Lisa Gabrielle Defazio" <ldefazio at stanford.edu> wrote in message
news:Pine.GSO.4.44.0208091028230.8153-100000 at saga21.Stanford.EDU...
>
> Does anyone have suggestions on how to prevent my protein from sticking to
> the membrane when I dialyze it?  I'm already adding loads of BSA, and my
> buffer contains 10% glycerol.
>
> Thanks,
> Lisa
>





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