sticky protein

miler-m at zahav.net.il miler-m at zahav.net.il
Tue Aug 13 07:22:10 EST 2002


Artem Evdokimov wrote:

> Detergents, nonionic for preference. BOG sometimes works miracles, but it is
> just one out of thousands of other possibilities. Watch out for micelle
> overconcentration.

> A.G.E.
> "Lisa Gabrielle Defazio" <ldefazio at stanford.edu> wrote in message
> news:Pine.GSO.4.44.0208091028230.8153-100000 at saga21.Stanford.EDU...
> >
> > Does anyone have suggestions on how to prevent my protein from sticking to
> > the membrane when I dialyze it?  I'm already adding loads of BSA, and my
> > buffer contains 10% glycerol.
> >
> > Thanks,
> > Lisa
>

No sticky protein using GeBAflex-tube Protein precipitation protocols see websites 
www.geba.org  for  Protein, and handbook details





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