sticky protein

[Mikhail Sinev]

Variation of ionic strength is the other option. Remove salt at all , for
beginning.


"Lisa Gabrielle Defazio" <ldefazio at stanford.edu> wrote in message
news:Pine.GSO.4.44.0208091028230.8153-100000 at saga21.Stanford.EDU...
>
> Does anyone have suggestions on how to prevent my protein from sticking to
> the membrane when I dialyze it?  I'm already adding loads of BSA, and my
> buffer contains 10% glycerol.
>
> Thanks,
> Lisa
>