sticky protein

Sandip Vyas feral at cats.ucsc.edu
Thu Aug 22 23:23:45 EST 2002


Before dialysis, I boil my membrane in EDTA (a pinch in a litre of water).
Hope this takes care of your sticky [protein] situation.

Sandip

   __o      __O                                        __@
  `\<,     `\<,                                       `\<,
(*)/(*)  (*)/(*)                                    (*)/(*)
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Bike to work, work to live, live to bike ... eat, drink, sleep.


"Lisa Gabrielle Defazio" <ldefazio at stanford.edu> wrote in message
news:Pine.GSO.4.44.0208091028230.8153-100000 at saga21.Stanford.EDU...
>
> Does anyone have suggestions on how to prevent my protein from sticking to
> the membrane when I dialyze it?  I'm already adding loads of BSA, and my
> buffer contains 10% glycerol.
>
> Thanks,
> Lisa



More information about the Proteins mailing list