stripping and western blot?

leezuyoug leezuyoug at yahoo.com
Fri Aug 23 08:43:51 EST 2002


Hi, I have met some problems in my stripping of PVDF membrane. I use
the protocol as follows:  50ml 62.5mM Tris-HCl (62.5), 2% SDS, 100mM
2-mecaptoethanol under 50C for 30 minutes. After stripping, wash 6x 5
minutes, then reprob again. But it is always blank without any
signal!!!
    I am working at p38 which is one kind of MAPK, using the ECL
reagent system.  Is there anyone can help me about this?
     thinks a lot!!!



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