Help: in vitro peptide synthesis
spal at iss.it
Tue Aug 27 03:38:43 EST 2002
Hello to all of you!
Does anybody have experience with in vitro peptide synthesis? I'm using the
Promega TNT-T7 Quick coupled translation-transcription kit in order to
obtain the protein translation of PCR fragments from human genomic DNA (PTT,
protein truncation test; size fragments: about 1300 bp; gene: exon 11 of
Each forward primer contains the T7 promoter sequence and a eukaryotic
translation initiation sequence. After synthesis, the products are analyzed
by 12% SDS-PAGE and autoradiography.
1) the size of translated peptide it's larger than expected (about 65 kD vs
2) an unexpected, high molecular weight peptide - about 85 kD - appears on
Any suggestion is welcome..
More information about the Proteins