stabilizing enzyme during storage

danark at t-online.de danark at t-online.de
Tue Aug 27 05:30:50 EST 2002


Now, can you tell me how to get rid of the glycerol after defrosting. Is the only way to dialyse??

Thx,
Daniel

Peter Cherepanov wrote:

> It is really not good to keep enzymes frozen at -20C.
> I red somewhere it has something to do with properities of salt solutions at
> these temperatures.

> Either add 50% glycerol and -20C (does not freeze).
> or add 10% glycerol, snap freeze and keep at -70C.






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