protein bound nucleotides

Guichy Waller waller at wi.mit.edu
Wed Aug 28 08:34:40 EST 2002


I am using a DEAE 650M colum (Toyopearl) to fractionate ATP<ADP and AMP.

I already tried to precipitate the proteins by treating the sample with
20% Acetnitrile, but am not sure it is working that well.
I would appreciate if someone can give me more ideas as of how to
separate the proteins from bound nucleotides before loading the sample
onto the column.
Thanks in advance,
Guichy




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