stripping and western blot?

Schwabe, TME tatjana.schwabe at ruhr-uni-bochum.de
Thu Aug 29 08:30:56 EST 2002


>>Hi, I have met some problems in my stripping of PVDF membrane. 


Hi,

we ususally strip by incubating the blot for 30 mins in 100 mM 
glycine-HCl pH 1.9, then block again w/ milk powder. This works fine 
with ECL-detection. You should be able to run 3 detections on the same 
blot (I have never done more, so I wouldn't know how often you can strip 
and block).

Hope this helps,

Tatjana






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