stripping and western blot?
tatjana.schwabe at ruhr-uni-bochum.de
Thu Aug 29 08:30:56 EST 2002
>>Hi, I have met some problems in my stripping of PVDF membrane.
we ususally strip by incubating the blot for 30 mins in 100 mM
glycine-HCl pH 1.9, then block again w/ milk powder. This works fine
with ECL-detection. You should be able to run 3 detections on the same
blot (I have never done more, so I wouldn't know how often you can strip
Hope this helps,
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