stripping and western blot?
tatjana.schwabe at ruhr-uni-bochum.de
Fri Aug 30 11:12:36 EST 2002
> Seems interesting. I never heard of using low pH in Westerns, although it is
> widely used elsewhere. The only thing I wonder is how you make glycine
> buffer pH 1.9, when none of the conventional pH meters can accurately
> measure such a value? Could you post you method or the source here?
Oops, I think you caught me on something there...
Basically we just titrate with HCl to what our pH-meter calls pH 1.9. I
just checked with one of the little pH-indicator strips and the pH is
somewhere between 1 and 2. I'd say it doesn't matter so much. I know
that in other protocols you elute AB with pH 3, so this should probably
suffice in Westerns as well.
I don't have a source for this protocol though, sorry.
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