protein precipitation in red blood cell lysates

mohammedmalaki at hotmail.com mohammedmalaki at hotmail.com
Mon Dec 2 13:32:11 EST 2002


Im trying to measure an enzymatic activity in red cell lysates. it involves measuring citrulline as a product using a spectrphometer. however, because of the high haemoglobin content there is interference with the absorbance readings. 
can anyone advise me how to get rid of the protein effect without affecting my enzyme activity?


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