Imaging zinc stained gels

Kyle Legate legatek at mcmail.cis.mcmaster.ca
Wed Dec 4 12:33:43 EST 2002


On 3 Dec 2002 tjmurray at bu.edu wrote:

> I am looking for the best way to capture an image of my zinc stained SDS-PAGE gel.  I have tried imaging it on a black background using the lab digital camera over a light box but the results were a little disappointing - a little fuzzy and problems with light leakage.   Do I need to use a densitometer for this?  I'm a little confused how this would work since this is a negative stain so the gel is semi-opaque with clear areas where the proteins are located.  Any help appreciated!
>
Dry it and scan it using a regular scanner.

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legatek at mcmaster.ca		Kyle Legate            legatek at hotmail.com

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