Imaging zinc stained gels
ajlmarques at hotmail.com
Mon Dec 9 13:36:04 EST 2002
Even better, don't dry it... just scan it with a regular scanner, placing
the gel between two transparent sheets...
"Kyle Legate" <legatek at mcmail.cis.mcmaster.ca> wrote in message
news:Pine.SOL.4.33.0212041233020.19554-100000 at mcmail.cis.mcmaster.ca...
> On 3 Dec 2002 tjmurray at bu.edu wrote:
> > I am looking for the best way to capture an image of my zinc stained
SDS-PAGE gel. I have tried imaging it on a black background using the lab
digital camera over a light box but the results were a little
disappointing - a little fuzzy and problems with light leakage. Do I need
to use a densitometer for this? I'm a little confused how this would work
since this is a negative stain so the gel is semi-opaque with clear areas
where the proteins are located. Any help appreciated!
> Dry it and scan it using a regular scanner.
> ... . . . . . . . . . . . . . . .
> legatek at mcmaster.ca Kyle Legate legatek at hotmail.com
> Tower of Tongues:Thursday PM:10:30-11:30 EDT:http://cfmu.mcmaster.ca
> moon musick:ritual:IDM:experimental(electronica):minimalism:glitch
> . . . . . . . . . . . . . . . ...
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