groel copurification( masters needed)
dk at no.email.thankstospam.net
Wed Dec 11 10:40:08 EST 2002
In article <20021210172019.2430.qmail at ww02.hostica.com>, cyril.boyault at ujf-grenoble.fr wrote:
>I NEED help.
>I copurify groel with an histagged protein. The interaction between groel and
> my protein is quite
>high because it resit in all buffers tested ( atp containing buffers, high
> glycerin ones, high salt,
>So if anybody has even a tiny suggestion....
The standard advice is to incubate in and then wash the column with
Mg/ATP to dissociate chaperones.
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