groel copurification( masters needed)

[D.K.]

In article <20021210172019.2430.qmail at ww02.hostica.com>, cyril.boyault at ujf-grenoble.fr wrote:
>Dear all,
>I NEED help. 
>I copurify groel with an histagged protein. The interaction between groel and
> my protein is quite 
>high because it  resit in all buffers tested ( atp containing buffers, high
> glycerin ones, high salt, 
>etc). 
>So if anybody has even a tiny suggestion....  
>

The standard advice is to incubate in and then wash the column with 
Mg/ATP to dissociate chaperones.

DK