groel copurification( masters needed)

D.K. dk at
Wed Dec 11 10:40:08 EST 2002

In article <20021210172019.2430.qmail at>, cyril.boyault at wrote:
>Dear all,
>I NEED help. 
>I copurify groel with an histagged protein. The interaction between groel and
> my protein is quite 
>high because it  resit in all buffers tested ( atp containing buffers, high
> glycerin ones, high salt, 
>So if anybody has even a tiny suggestion....  

The standard advice is to incubate in and then wash the column with 
Mg/ATP to dissociate chaperones.


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