AEVDOKIMOZ at cinci.rr.com
Sun Dec 15 12:49:48 EST 2002
<wqpgunman at 163.com> wrote in message
news:20021215131357.31314.qmail at ww02.hostica.com...
> my recombinant enzyme becomes smaller in E.coli and has activity in
DH5¦Á,but has no activity in M15.who can help me?
Help you with what ? You seem to have a case of host-dependent proteolysis
going, but without some details regarding how you cloned your stuff, what
the stuff is, what have you already tried, etc. we can't really help you
apart from generic solutions such as 'change host cells' and 'clone the
protein as a fusion' etc.
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