what the inclusion body pellets look like

fxiong at asu.edu fxiong at asu.edu
Wed Dec 18 01:55:17 EST 2002

Hi, all, 

I recently extracted overexpressed protein which was expressed probably in the form of inclusion body. I break the E. coli cells and spin down the pellet. It seemed kinds of difficulty to resuspend the pellet. Also, the suspensions are pretty jelly-like (Viscosity problem?) I'm wondering is this normal for inclusion body isolation?         


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