what the inclusion body pellets look like

Artem Evdokimov AEVDOKIMOZ at cinci.rr.com
Wed Dec 18 16:42:32 EST 2002


Most of the time dense inclusion bodies look kind of like very very fine
yellowish-white powder. Inclusion bodies should be soluble in certain
detergents and in strong denaturants. Resuspension of IB in simple buffers
usually results in clear buffer and IB pellet after centrifugation.
Various semi-folded forms of proteins can result in the effect(s) that you
describe. Some DNA-binding proteins also create a 'jelly' after lysis due to
crosslinking of DNA framgents.

A.G.E.
<fxiong at asu.edu> wrote in message
news:20021218065517.24337.qmail at ww02.hostica.com...
> Hi, all,
>
> I recently extracted overexpressed protein which was expressed probably in
the form of inclusion body. I break the E. coli cells and spin down the
pellet. It seemed kinds of difficulty to resuspend the pellet. Also, the
suspensions are pretty jelly-like (Viscosity problem?) I'm wondering is this
normal for inclusion body isolation?
>
>
>
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