what the inclusion body pellets look like

Kyle Legate legatek at mcmail.cis.mcmaster.ca
Wed Dec 18 16:30:12 EST 2002


On 18 Dec 2002 fxiong at asu.edu wrote:

> I recently extracted overexpressed protein which was expressed probably in the form of inclusion body. I break the E. coli cells and spin down the pellet. It seemed kinds of difficulty to resuspend the pellet. Also, the suspensions are pretty jelly-like (Viscosity problem?) I'm wondering is this normal for inclusion body isolation?
>
The jelly is likely DNA. If it bothers you, add some DNAse to break it up.

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legatek at mcmaster.ca		Kyle Legate            legatek at hotmail.com

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