Trouble removing contaminating proteins from amylose resin

Artem Evdokimov AEVDOKIMOZ at cinci.rr.com
Mon Feb 18 11:27:47 EST 2002


I wish they published more about the actual composition of the amylose resin
and the specific chemistry of the linker. The amylose resin is indeed
somewhat strange, however we have in the past used beta octyl glucoside (but
not dodecyl maltoside, for obvious reason!) with it and everything was OK.
In your case, if your parasite proteins bind really tightly then you
probably will end up with some serious additional purification - try HIC yet
?

Good luck,

Artem aka A.G.E.
"John Everett" <kellere at hotmail.com> wrote in message
news:5a7ca595.0202172204.466ceffe at posting.google.com...
> "Artem Evdokimov" <AEVDOKIMOZ at cinci.rr.com> wrote in message
news:<5dBb8.52974$Hu6.14207829 at typhoon.neo.rr.com>...
> > Tried nonionic detergents yet ? Glycerol/ethylene glycol ? Phosphate ?
Try
> > figuring out what the three free-riders are (MS) and add something
that'd
> > get rid of them.
> >
> > A.G.E.
>
>
> A.G.E:
>
>    Thanks for the idea.
>      I have been warned about amylose resin and detergents
>      but review of the literature that comes with the resin
>      tells me that I can use .2% Tween-20 or Triton-X-100.
>
>      --John





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