pKa of a His tag.

John Everett kellere at hotmail.com
Mon Feb 18 19:36:54 EST 2002


Artem,

   Thanks for the advice once again.
   The carboxy peptidase sounds interesting
   though I am already cloning in a thrombin
   clevage site adjacent to the His tag.
   The pH of 7.0 or less is indeed to prevent
   amide proton - solvent exchange when the
   sample is in the NMR.

      Thanks,
         John




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