pKa of a His tag.
AEVDOKIMOZ at cinci.rr.com
Mon Feb 18 20:03:52 EST 2002
Just remember that thrombin is promiscuous and can eat up your protein if
you have sequences that resemble thrombin cleavage site.
good luck !
"John Everett" <kellere at hotmail.com> wrote in message
news:5a7ca595.0202181636.b751328 at posting.google.com...
> Thanks for the advice once again.
> The carboxy peptidase sounds interesting
> though I am already cloning in a thrombin
> clevage site adjacent to the His tag.
> The pH of 7.0 or less is indeed to prevent
> amide proton - solvent exchange when the
> sample is in the NMR.
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