ECL ghost bands

Douglas Feinstein dlfeins at uic.edu
Tue Feb 19 17:27:59 EST 2002


I apologize if this has been posted previously. We get many negative 
images, or ghost bands, on our ECL exposures. Almost all the time we get 
these things with the MW standards that were used; and then with certain, 
but not all primaries. We're using PVDF, transfering in CAPS buffers, and 
using amersham's ECL reagents. I suspect we should drop primary and 
secondary amounts down a factor of 5 or 10 (now we use at 1:2000 primary; 
1:10,000 secondary

Doug Feinstein
1819 West Polk St
MC 519, Rm 544
Chicago, IL 60612

fax: 815 333 0449
voice: 312 355 1665
email: dlfeins at uic.edu


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