An old, never answered question: Qiagen's miniprep buffers - analytical chemistry anyone?

wo hubahopp at gmx.de
Thu Feb 21 04:27:55 EST 2002


Dima,

K+ and Cl- (as maybe phosphate, too) are easiest to determine by subjecting
a diluted aliquot to automated urine or serum analysis in the clinical
chemitsry lab of a hospital. Hoping that Gu doesn't interfere though.

K+ also should be able to be determined gravimetrically using SDS :) if
there are lots present (add excess NaSDS, filer through pre-weighted paper
or collect by centrifugation in pre weighted tube, wash precipitate with
NaSDS, allow to dry)

Assuming all volatiles present are water and ipropOH, one might distill the
stuff and measure density of the condensate comparing against a standard
curve.

what about acetate? might be concluded from potassium and sodium...

tris - the amino group should react with sangers reagent (dansyl chloride),
determine fluorescence or absorbance against standard curve. no idea if it
interferes with gunaidinium.

hacking regards,
Wo

>- Ac-
>- K+
>- 2-propanol
>- GuCl (as it is bound to be present at high concentrations, just
>  Cl- or even conductivity will probably be good enough)
> - Tris (amine reaction?)

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