Simple myoglobin digest question

Gerd Klaassen klaassen at coastweb.de
Fri Feb 22 06:53:04 EST 2002


Hello,

just a simple question:

Can myoglobin be digested with trypsin under
standard conditions (1:50 ratio, 37 C, 14h, TrisHCl pH 8,5)
without any denaturation (guanidine, SDS etc.)?

I tried horse heart myoglobin and found  - nothing, only
the myoglobin itself. Is it my trypsin or just the conditions?

Kind reagards,
Gerd Klaassen




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