Hello all, I'm having problems in detecting IL3 using IEF gels. IL3 is a polypeptide ~15.5 kDa with pI ~7. I can run standard SDS and see good bands with coomassie staining, but it doesn't work with IEF. Is there any way I can prevent IL3 from diffusing out of the IEF gel during the staining and destaining step? Thank you. Ahmad ---