Isoelectric focussing of peptides

Ahmad Yahya armyahya at engmail.uwaterloo.ca
Sat Feb 23 02:17:11 EST 2002


Hello all,

I'm having problems in detecting IL3 using IEF gels.  IL3 is a polypeptide
~15.5 kDa with pI ~7.  I can run standard SDS and see good bands with
coomassie staining, but it doesn't work with IEF.  Is there any way I can
prevent IL3 from diffusing out of the IEF gel during the staining and
destaining step?

Thank you.

Ahmad

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