Iron-binding proteins of Salmonella
AEVDOKIMOZ at cinci.rr.com
Thu Feb 28 22:57:08 EST 2002
Immunoblot. Affinity chromatography. If you have it, gene-chip arrays. It
all depends on how much effort and capital can you afford to spend on this.
Immunoblot sounds like the cheapest option.
<vetimmunol at hau.nic.in> wrote in message
news:18b4016ddd.16ddd18b40 at hau.nic.in...
> Dear all,
> I have encountered one problem. I want to study the expression of iron-
> (Fe3+) binding protein's profiles of Salmonella typhimurium grown under
> iron-sufficient and iron-rich conditions. Can anyone tell me how to
> study/or stain iron-binding proteins of Salmonella in SDS-PAGE gels? Is
> there any other method specifically for this? Any help would be greatly
> Sincere regards,
> Devender Kr. Saini,
> Immunodiagnosis Lab,
> Dept. Veterinary Parasitology,
> College of Veterinary Sciences,
> CCS Haryana Agricultural University, Hisar, Haryana,
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