biotinylated 80bp-Fragment

user q6252397 at fernuni-hagen.de
Tue Jan 8 10:49:59 EST 2002


Dear all.

I would like to purify a transcription factor with DNA-Affinity
chromatography.
I bought a Streptavidin-Superflow Agarose resin. I'd like to couple
biotinylated DNA-Fragments (80bp) on the column, to get active TF.
The resin has a Biotin binding capacity of 350 nmol/ml resin.
The problem is to get that much biotinylated DNA.

Do you have any ideas for that? Any comments would be appreciated.

TIA,

Tina





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