computing the pI of a phosphoprotein

Artem Evdokimov AEVDOKIMOZ at cinci.rr.com
Thu Jan 10 19:33:04 EST 2002


You are probably right. As far as calculating pI - i haven't looked
expliciltly, but you should be able to modify a simple pI calculation using
a 'non-standard' amino acid, such as phosophotyrosime serine, threonine or
some other unusual stuff. i bet someone coded this.

Artem
"Will Fuller" <will_fuller at hotmail.com> wrote in message
news:B86336F9.51BC%will_fuller at hotmail.com...
> Hi,
>
> I'm trying to get a rough estimate of the shift I should expect on a 2D
gel
> when my protein of interest is phosphorylated. It seems to me that the
shift
> would be bigger for a protein of, say 10kDa, than for one of say 100kDa.
Is
> this right? I know there are lots of resources available for calculating
> theoretical pI's of proteins, but do they also exist for phosphoproteins?
>
> I know there are other variables to consider as well as just the size of a
> protein, but I'd just like a rough rule of thumb. Thanks in advance.
> Will
>
>
> ---





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