overexpressed protein sometime gives two bands

user q6252397 at fernuni-hagen.de
Tue Jan 15 16:06:21 EST 2002


Dear all,

I'm overexpressing different proteins (phage, E. cloacae and C. freundii,
with and without his-Tag) in E. coli.
Usually I have a good expression and a single band during purification.
I wonder why I sometimes get two band, which are there all the time during
purification. They differ only very little in Mw. Probably much less than 5
kD.
I suppose, it is the same protein because it seems to bind on the column
exactly like the "one-band-protein".

Any explanations for this?

TIA,
Tina





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