Soluble GFP???

Frank Fürst ffrank at rz.uni-potsdam.de
Thu Jan 17 05:32:32 EST 2002


rhunte01 at uoguelph.ca (Ryan Hunter) schrieb:

> Hi everybody reading this....
> 
> Is there such a thing as secretory GFP or a soluble form of it that 
> doesnt aggregate?  Im looking to do some simple chemical analyses but I'd 
> need a soluble form to do them.

I've heard many people doing mutational studies (varying
fluorescence...) or even using GFP-fusion proteins, and they never
complained about problems with solubility. On the contrary, GFP seems to
be really stable, R. Glockshuber (ETH Zürich) did fluorescence studies
under conditions where the fusion partner was denatured by Guanidine,
IIRC. 

The folding of GFP, however, seems to be kind of problematic, since it
has been used as a model substrate of GroEL (see, e.g., J. Weissman's
homepage at http://www.hhmi.org/research/investigators/weissman.html). I
never bothered about the expression systems these people used.


Bye, Frank




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